NANOEXTRACT KAEMPFERIA GALANGA ANTIOXIDANT AND ANTIBACTERIAL EFFECTIVENESS AGAINST PORPHYROMONAS GINGIVALIS

SHERLEY GANDA (2023) NANOEXTRACT KAEMPFERIA GALANGA ANTIOXIDANT AND ANTIBACTERIAL EFFECTIVENESS AGAINST PORPHYROMONAS GINGIVALIS , TESIS, UNIVERSITAS PRIMA INDONESIA

ABSTRAK

Dental biofilm, with major pathogens of Porphyromonas gingivalis has been identified  to be reservoir for periodontal disease. Chlorhexidine gluconate 0.2 %, and amoxicillin with metronidazole has been widely used as an adjunct to treat, however  causing  numerous adverse effects.  Kencur (Kaemferia Galanga) in form of extract and nanoparticle is studied  to evaluate optimal concentration of  antioxidant capacity and  to compare the effectiveness of inhibition  to P. gingivalis.  Nano samples are obtained from galanga extract, mixed with sodium tripoliphsophate 0.4%, and  chitosan solution  1% using ionic gelation method, to ensure the suspension is stable and to prevent aggregation.  Both forms indicate optimal antioxidant activity in  concentration of 6,25µg/ml  shown by DPPH scavenging activities and ABTS reduction test. Comparison were made between them to determine its viability, in terms of minimum bacterial concentration, as well as   minimum inhibitory.   The study was analysed statistically using  normality, homogeneity,  followed by Post Hoc Tukey HSD Test, and the results shows statistically difference (p <0.05, CI 95%), with nanogalanga exhibits better antibacterial activity compared to the extract. Positive correlation is found  between  inhibitory rate and   concentration, the  higher the concentration, the higher the inhibition is.

 Keywords : Kaemferia galanga, Porphyromonas gingivalis, chitosan nanoparticle, ionic gelation

 

 

JURNAL
KATEGORI JURNAL Jurnal Internasional Bereputasi
TAHUN JURNAL 2023
VOLUME JURNAL 20
NOMOR JURNAL 1
NAMA PENERBIT Journal of Medicinal and Aromatic Plants
NOMOR ISSN/ISBN 0974-7877
LAMAN PENERBIT (URL) https://epubs.icar.org.in/index.php/JMAP
LAMAN ARTIKEL (URL) https://epubs.icar.org.in/index.php/JMAP