Acne or acne vulgaris is a common skin condition caused by clogging of hair follicles or pores of oily skin. Suji (Dracaena angustifolia [Medik.] Roxb.), an Indonesian herbal plant, is used to prevent bacterial growth. The study was conducted to identify the phytochemicals of ethanol extract of suji leaves and analyze the cytotoxic and antibacterial properties against Propionibacterium acnes and Staphylococcus aureus of its nanoemulsion. The results showed that the ethanol extract of suji leaves contained active compounds of alkaloids, phenols, flavonoids, saponins, triterpenoids, and steroids which are secondary metabolites. Serum nanoemulsion showed strong antibacterial activity against Propionibacterium acnes and Staphylococcus aureus at 500 and 750 ppm.  The IC50 value of MTT Assay for the cytotoxic effect of nanoemulsion of ethanol extract of suji leaves was 61.92 µg/mL, which indicated that the extract was moderately active against prepuce cells, which is used as a model of acne prone skin. Nanoemulsion of suji leaf ethanol extract has antibacterial and cytotoxic properties, so it has potential as an anti-acne agent.