MOLECULAR IDENTIFICATION OF ENZYME-PRODUCING THERMOPHILIC BACTERIA ISOLATED FROM GEOTHERMAL HOTSPRING IN SIMBOLON VILLAGE, NORTH SUMATERA, INDONESIA

DIAN HARDIYANTI ROSDA (2021) MOLECULAR IDENTIFICATION OF ENZYME-PRODUCING THERMOPHILIC BACTERIA ISOLATED FROM GEOTHERMAL HOTSPRING IN SIMBOLON VILLAGE, NORTH SUMATERA, INDONESIA , TESIS, UNIVERSITAS PRIMA INDONESIA

ABSTRAK

Indonesia has many natural resources, but not all are well utilized, including thermophilic bacteria, which have not been fully explored. Thermophilic bacteria live in a location with a temperature of 45ᴼC to 70ᴼC, such as hot springs or volcanic craters. Thermophilic, high-temperature resistant bacteria are very efficient in producing enzymes and can also be cultivated quickly and in large quantities with high stability at high temperatures. Thermophilic bacteria have the potential to produce thermophilic enzymes that can stabilize against hot temperatures. Generally, enzymes will be damaged against high temperatures. Enzyme isolation from thermophilic bacteria is beneficial in various fields, such as in the industry, which is almost entirely in using high temperatures. These enzymes have many advantages because they can increase the reaction to save production costs, energy, and time in the industrial process. Thermophilic bacteria are found in many places, such as areas with volcanic activity and also hot springs. As a result, it can be concluded that UTMSBA and UTMSBS isolates showed positive results in the gelatin hydrolysis test, while the citrate test, catalase test, sulfide hydrolysis, gelatin hydrolysis, and mortality test showed negative results. In terms of the shape, the edges and elevations of UTMSBA and UTMSBS isolates appear circular, entire, and flat. Similarly, the isolates can hydrolyze amylase, cellulase, and protease enzymes, and the results of identifying the isolate with the 16SrRNA gene showed 99.99% homology, namely Bacillus amyloliquefaciens strain V4.

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