ANTIOXIDANT AND WOUND HEALING POTENTIAL OF PERSEA AMERICANA MILL LEAVES EXTRACT

WILDA HAYATI (2021) ANTIOXIDANT AND WOUND HEALING POTENTIAL OF PERSEA AMERICANA MILL LEAVES EXTRACT , TESIS, UNIVERSITAS PRIMA INDONESIA

ABSTRAK

Persea Americana Mill. (avocado) is the dicotyledonous plant that rich in secondary metabolite compounds such as saponins, alkaloids, terpenoids, and flavonoids. It applies as an antioxidant, anti-inflammatory, anti- lithiasis, and anti-diabetic. This study investigates the wound healing properties of P. americana leaves extract on Mus musculus in gel formulations. Preliminary phytochemical screening had done qualitatively, and antioxidant capacity was evaluated by the DPPH scavenging method. Extract in gel formulation contained various concentration of extracts (1%, 3%, 5%, and 7% v/v) was performed to observed the wound healing activity compared to standard medicine Bioplaceton® on mice. The phytochemical test showed the presence of alkaloids, flavonoids, saponins, and tannins. The result of the DPPH scavenging activity (IC50) of the leaves extract was 8.92 ppm. In wound healing activity, the application of extract P. americana leaves with various concentrations (1%, 3% , 5%, and 7% v/v) in gel formulations showed decreasing of wound length drastically to 0.9, 0.3, 0.2, and 0.7 cm, respectively. Gel- 5% extract showed the highest percentage on wound healing, 90%, where gel-1% extract was 55%, gel-3% extract was 85%, and gel-7% extract was 75%. The 5% extract gel formulations showed a significant result similar to Bioplacenton®, with the percentage of wound healing being 90%. It can be concluded that the extract of P. americana leaves could be the potential for natural antioxidant and wound healing sources in the future.

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