PHYTOCHEMICAL AND ANTIBACTERIAL ANALYSIS OF THE FORMULATED CREAM OF BLACK CUMIN HONEY

IMELIA WIJAYA (2021) PHYTOCHEMICAL AND ANTIBACTERIAL ANALYSIS OF THE FORMULATED CREAM OF BLACK CUMIN HONEY , TESIS, UNIVERSITAS PRIMA INDONESIA

ABSTRAK

Black cumin honey is produced by bees that feed on the nectar of black cumin flower. Both honey and black cumin have been known for their benefits for centuries.  This study aims to analyze the bioactive compound of black cumin honey and to investigate the antibacterial activity of its formulated cream against Propionibacterium acnes and Pseudomonas aeruginosa. HPLC and GC-MS method were used to analyze the bioactive compounds of the black cumin honey.The formulated cream of black cumin honey was prepared in an oil-in-water type cream with various concentrations of 1%, 5%, 10%, 15%, 20%, and 30% (w/w). Antibacterial effects against Propionibacterium acnes and Pseudomonas aeruginosa were examined by the disc diffusion method. Physical stability of formulated cream included organoleptic, homogeneity, and pH measurement test. Result of GC-MS analysis of black cumin honey showed 25 compounds; among those were organic fatty acids, saccharides, and amino acid. HPLC analysis of flavonoid content showed quercetin as the bioactive compound at a concentration of 0.223 mg/g. The physical stability test showed that black cumin honey cream was physically stable. Disc diffusion analysis showed great inhibitory potential against P. acnes and P. aeruginosa in 30% concentration with d = 11.2 mm and 10.8 mm, respectively. It can be concluded that the formulated cream of black cumin honey showed a great potential application as antibacterial agents of P. acnes and P. aeruginosa.

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